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In vitro evaluation of calcium alginate gels as matrix for iontophoresis electrodes.

The method should be useful for detecting calcium-binding proteins which may trigger neurotransmitter release. In addition this method will detect at least one example from every described class of calcium-binding protein, including lectins and γ-carboxyglutamic acid containing calcium-binding proteins. Their efforts have produced a method which is shorter, has 40-fold greater sensitivity over the previous method, and will detect 'EF hand'-containing calcium-binding proteins in polyacrylamide gels below the 0.5 μg level. The authors refined the method of Schibeci and Martonosi (1980) to enhance detection of calcium-binding proteins in polyacrylamide gels using 45 Ca 2+. International Nuclear Information System (INIS) Improved detection of calcium-binding proteins in polyacrylamide gels